This guide gives you a comprehensive overview of the 18234 ELISA kit. It’s designed to quantify a specific analyte, like a protein or hormone. If you’re a researcher, lab technician, or clinical scientist, this is for you.
The kit is commonly used in fields like immunology, oncology, and neuroscience. I’ll cover the essential details—specifications, protocol, and components. This will help you quickly figure out if it fits your research needs.
Core Function and Target Analyte Details
The 18234 ELISA kit detects a target analyte known as Interleukin-6 (IL-6). It’s also commonly referred to as B-cell stimulatory factor-2 (BSF-2) or interferon beta-2.
IL-6 is a cytokine that plays a crucial role in the immune response and inflammation. In normal physiological processes, it helps regulate the immune system and supports the body’s defense against infections. However, when overproduced, it can contribute to chronic inflammation and various diseases like rheumatoid arthritis and cancer.
This kit has been validated for use with Human, Mouse, and Rat samples. That’s pretty handy if you’re working across different model organisms.
Measuring IL-6 can help researchers understand the underlying mechanisms of inflammatory diseases and develop new therapeutic strategies. Clinicians can also use it to monitor disease progression and treatment efficacy.
The 18234 ELISA kit is for research use only (RUO). This is important to know, especially if you’re considering using it in a clinical setting.
Quantifying IL-6 is vital because it provides insights into the inflammatory state of an organism. It helps us better understand and manage conditions where inflammation is a key factor.
Key Performance Specifications and Data
When you’re working with an ELISA kit, knowing the quantitative detection range is crucial. For the 18234 elisa, it can accurately measure concentrations from 15 pg/mL to 1000 pg/mL.
The sensitivity or Limit of Detection (LOD) is another key metric. This kit can distinguish the analyte at a minimum of 15 pg/mL.
What about sample types? The 18234 elisa works well with serum, plasma, cell culture supernatants, tissue homogenates, and urine.
Precision is everything in research. The intra-assay coefficient of variation (CV%) for this kit is typically around 5%, and the inter-assay CV% is around 8%. This shows good reproducibility.
Time is money, especially in the lab. The total assay time for the 18234 elisa is about 2.5 hours. This helps in planning your workflow efficiently.
Lastly, the required sample volume per well is 100 μL. This is important for planning your sample collection and dilution strategy.
Assay Principle and Protocol Overview
Let’s dive into the 18234 ELISA, a Sandwich ELISA. This format is like a sandwich, where the target analyte is captured between two layers of antibodies.
First, the plate is coated with a specific antibody. When you add your sample, the target protein binds to this antibody. Then, you add a detection antibody that binds to the target protein.
The detection method here is colorimetric. You’ll see a change in color, which you can measure at a specific wavelength. It’s like watching a painting come to life as the colors develop. Alletomir
Standard preparation is key. You need to make sure your standards are accurate to create a reliable curve. Incubation times are also critical.
Too short, and you might miss some binding. Too long, and you could get false positives.
Wash steps are essential. They help remove any unbound proteins, making sure only the target is detected. Think of it as cleaning up after a party—only the guests (target proteins) should be left behind.
Substrate addition is the final step. The substrate reacts with the detection antibody, producing the color change. It’s like adding the finishing touches to a masterpiece.
One unique feature of this protocol is the pre-coated plates. This saves time and reduces the chances of errors. Plus, ready-to-use reagents make the process smoother, like having all your ingredients pre-measured for a recipe.
Kit Components and Storage Requirements
When you get your 18234 ELISA kit, it comes with everything you need to start your experiment. The main components include a 96-well microplate, standards, detection reagents, wash buffer, and substrate.
But here’s the thing: what about the stuff that’s not in the box? You’ll need a microplate reader, pipettes, and distilled water. Do you have those ready?
Now, let’s talk storage. Once you receive the kit, store it at 4°C. This keeps everything in top shape.
How long will the kit last? When stored correctly, the shelf life is typically around six months. But once you open it, some components might need to be kept at -20°C.
Always check the instructions for each part.
Before you dive into your experiment, make sure to check the expiration date on the box and reagents. The last thing you want is to run an experiment with expired materials, right?
Next Steps for Your 18234 ELISA Assay
The 18234 elisa kit offers high sensitivity and specificity, ensuring accurate and reliable results. Its validated performance makes it a trusted choice for researchers.
Ideal for specific research needs, this kit is particularly valuable in applications requiring precise quantification of target analytes.
For more detailed information, refer to the full product manual or datasheet.
To find pricing, availability, or to request a quote, visit our website’s product page.
For any specific questions about protocol optimization or troubleshooting, contact our technical support team or visit the FAQ page.
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